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Is Leishmania (Viannia) peruviana a distinct species? A MLEE/RAPD evolutionary genetics answer

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Show simple item record Bañuls, A. L. en_US Dujardin, J. C. en_US Guerrini, F. en_US De Doncker, S. en_US Jacquet, D. en_US Arevalo, J. en_US Noël, S. en_US Le Ray, D. en_US Tibayrenc, M. en_US 2007-12-06T14:33:10Z 2007-12-06T14:33:10Z 2000 en_US
dc.identifier.issn 1066-5234 en_US
dc.identifier.other ITG-P2A en_US
dc.identifier.other ITG-P4B en_US
dc.identifier.other ITG-P5B en_US
dc.identifier.other ITG-P8A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other Population genetics en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract A set of 38 Leishmania stocks from the Andean valleys of Peru was characterized by both Multilocus Enzyme Electrophoresis (MLEE) and Random Amplified Polymorphic DNA (RAPD). Data were analyzed in terms of taxonomy and evolutionary genetics. Synapomorphic MLEE and RAPD characters, clear-cut clustering, and strong agreement between the phylogenies inferred from either MLEE or RAPD supported the view that Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis correspond to two closely related, but distinct monophyletic lines (clades) and can therefore be considered as "discrete typing units" (DTUs). The question whether the L. (V.) peruxviana DTU deserves species status is dependent upon the desirability of it, in terms of epidemiological and medical relevance. A previous Orthogonal Field Alternating Gel Electrophoresis (OFAGE) analysis of the same L. (V.) peruviana isolates was published by Dujardin et al. (1995b). The data from the different markers (i.e. MLEE, RAPD and OFAGE) were compared by population genetics analysis. RAPD and OFAGE provided divergent results, since RAPD showed a strong linkage disequilibrium whereas OFAGE revealed no apparent departure from panmictic expectation. MLEE showed no linkage disequilibrium. Nevertheless, contrary to OFAGE, this is most probably explainable by the limited variability revealed by this marker in L. (V.) peruviana (statistical type II error). RAPD data were consistent with the hypothesis that the present L. (V.) peruviana sample displays a basically clonal population structure with limited or no genetic exchange. Disagreement between RAPD and OFAGE can be explained either by accumulation of chromosomal rearrangements due to amplification/deletion of repeated sequences, or by pseudo-recombinational events. en_US
dc.language English en_US
dc.subject Protozoology en_US
dc.subject Leishmania peruviana en_US
dc.subject Characterization en_US
dc.subject MLEE en_US
dc.subject RAPD en_US
dc.subject Taxonomy en_US
dc.subject Genetics en_US
dc.subject Evolutionary genetics en_US
dc.title Is Leishmania (Viannia) peruviana a distinct species? A MLEE/RAPD evolutionary genetics answer en_US
dc.type Article en_US
dc.citation.jtitle Journal of Eukaryotic Microbiology en_US
dc.citation.volume 47 en_US
dc.citation.pages 197-207 en_US
dc.citation.jabbreviation J Eukar Microbiol en_US

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