Institute of Tropical Medicine Antwerp
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Recent progress in molecular diagnosis of sleeping sickness

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dc.contributor.author Deborggraeve, S.
dc.contributor.author Büscher, P.
dc.date.accessioned 2013-02-13T12:52:32Z
dc.date.available 2013-02-13T12:52:32Z
dc.date.issued 2012
dc.identifier.issn 1473-7159
dc.identifier.doi http://dx.doi.org/10.1586/erm.12.72
dc.identifier.other ITG-B1A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-PARDIA
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD54
dc.identifier.uri http://hdl.handle.net/10390/7315
dc.description.abstract This article will review the most recent progress in the molecular diagnosis of sleeping sickness and its potential role in patient management and disease control. While PCR remains restricted to research and reference laboratories, promising alternative molecular platforms have emerged over the last few years. Several loop-mediated isothermal amplification assays have been developed for detection and identification of the parasite with reported high analytical sensitivity and specificity. Simplified loop-mediated isothermal amplification formats have been designed and are undergoing evaluation studies in the field. Accurate diagnosis based on specific detection of the parasite's ribosomal RNA has been made possible by the isothermal nucleic acid sequence-based amplification and by direct hybridization with fluorescent detection probes. In addition to the technological progress, the authors also discuss the diagnostic performance of molecular tests in the most recent clinical evaluation studies and briefly present some viewpoints for the near future. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Sleeping sickness en
dc.subject Trypanosoma brucei gambiense en
dc.subject Trypanosoma brucei rhodesiense en
dc.subject Vectors en
dc.subject Tsetse flies en
dc.subject Glossina morsitans en
dc.subject Diagnosis en
dc.subject Molecular diagnostic techniques en
dc.subject Polymerase chain reaction en
dc.subject PCR en
dc.subject Amplification en
dc.subject Ribosomal DNA en
dc.subject Nucleic acids en
dc.subject Sequence analysis en
dc.subject Fluorescent antibody technique en
dc.subject Fluorescence microscopy en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Laboratory techniques and procedures en
dc.subject Review of the literature en
dc.title Recent progress in molecular diagnosis of sleeping sickness en
dc.type Article en
dc.citation.issue 7 en
dc.citation.jtitle Expert Review of Molecular Diagnostics en
dc.citation.volume 12 en
dc.citation.pages 719-730 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/23153239
dc.citation.jabbreviation Expert Rev Mol Diagn en


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