Institute of Tropical Medicine Antwerp
Foundation of Public Utility

A catalogue of putative HIV-1 protease host cell substrates

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Show simple item record Impens, F. Timmerman, E. Staes, A. Moens, K. Ariën, K. K. Verhasselt, B. Vandekerckhove, J. Gevaert, K. 2013-03-29T13:55:08Z 2013-03-29T13:55:08Z 2012
dc.identifier.issn 1431-6730
dc.identifier.other ITG-B5A
dc.identifier.other DBM
dc.identifier.other U-VIROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD55
dc.description.abstract Processing of human immunodeficiency virus (HIV) proteins by the HIV-1 protease is essential for HIV infectivity. In addition, several studies have revealed cleavage of human proteins by this viral protease during infection; however, no large-scale HIV-1 protease degradomics study has yet been performed. To identify putative host substrates in an unbiased manner and on a proteome-wide scale, we used positional proteomics to identify peptides reporting protein processing by the HIV-1 protease, and a catalogue of over 120 cellular HIV-1 protease substrates processed in vitro was generated. This catalogue includes previously reported substrates as well as recently described interaction partners of HIV-1 proteins. Cleavage site alignments revealed a specificity profile in good correlation with previous studies, even though the ELLE consensus motif was not cleaved efficiently when incorporated into peptide substrates due to subsite cooperativity. Our results are further discussed in the context of HIV-1 infection and the complex substrate recognition by the viral protease. en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV-1 en
dc.subject AIDS en
dc.subject Infectivity en
dc.subject Proteins en
dc.subject Protease en
dc.subject Proteomics en
dc.subject Peptides en
dc.subject Specificity en
dc.title A catalogue of putative HIV-1 protease host cell substrates en
dc.type Article en
dc.citation.issue 9 en
dc.citation.jtitle Biological Chemistry en
dc.citation.volume 393 en
dc.citation.pages 915-931 en
dc.citation.jabbreviation Biol Chem en

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