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M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A

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dc.contributor.author Ibañez, L. I. en_US
dc.contributor.author Roose, K. en_US
dc.contributor.author De Filette, M. en_US
dc.contributor.author Schotsaert, M. en_US
dc.contributor.author De Sloovere, J. en_US
dc.contributor.author Roels, S. en_US
dc.contributor.author Pollard, C. en_US
dc.contributor.author Schepens, B. en_US
dc.contributor.author Grooten, J. en_US
dc.contributor.author Fiers, W. en_US
dc.contributor.author Saelens, X. en_US
dc.date.accessioned 2014-09-25T13:39:19Z
dc.date.available 2014-09-25T13:39:19Z
dc.date.issued 2013 en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0059081 en_US
dc.identifier.other ITG-B7B; DBM; U-VIROL; JIF; DOI; FTA; E-only; Abstract; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/7567
dc.description.abstract The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4(+) T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection. en_US
dc.language English en_US
dc.subject Viral diseases en_US
dc.subject Influenza en_US
dc.subject Research en_US
dc.subject Vaccine development en_US
dc.subject Immune response en_US
dc.subject Proteins en_US
dc.subject Nucleic acids en_US
dc.subject Immunogenicity en_US
dc.subject Efficacy en_US
dc.subject Vaccination en_US
dc.subject Antibodies en_US
dc.subject CD4-positive-T-lymphocytes en_US
dc.subject In vivo en_US
dc.subject Mice en_US
dc.subject Laboratory techniques and procedures en_US
dc.title M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A en_US
dc.type Article-E en_US
dc.citation.issue 3 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.volume 8 en_US
dc.citation.pages e59081 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/23527091 en_US
dc.citation.jabbreviation PLoS ONE en_US


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