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A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for Trypanosoma brucei gambiense

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dc.contributor.author Van Nieuwenhove, L. en_US
dc.contributor.author Büscher, P. en_US
dc.contributor.author Balharbi, F. en_US
dc.contributor.author Humbert, M. en_US
dc.contributor.author Guisez, Y. en_US
dc.contributor.author Lejon, V. en_US
dc.date.accessioned 2014-09-25T13:39:36Z
dc.date.available 2014-09-25T13:39:36Z
dc.date.issued 2013 en_US
dc.identifier.issn 1360-2276 en_US
dc.identifier.doi http://dx.doi.org/10.1111/tmi.12058 en_US
dc.identifier.other ITG-B1B; ITG-B2A; ITG-B3B; ITG-BLA; DBM; U-PARDIA; JIF; DOI; Abstract; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/7752
dc.description.abstract OBJECTIVE: To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG) LiTat 1.5 amino acid (AA) sequence 268-281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of Trypanosoma brucei gambiense sleeping sickness. METHODS: A synthetic biotinylated peptide (peptide 1.5/268-281), native VSG LiTat 1.3 and VSG LiTat 1.5 were tested in an indirect ELISA with 102 sera from patients with HAT and 102 endemic HAT-negative controls. RESULTS: The area under the curve (AUC) of peptide 1.5/268-281 was 0.954 (95% confidence interval 0.918-0.980), indicating diagnostic potential. The areas under the curve of VSG LiTat 1.3 and LiTat 1.5 were 1.000 (0.982-1.000) and 0.997 (0.973-1.000), respectively, and significantly higher than the AUC of peptide 1.5/268-281. On a model of VSG LiTat 1.5, peptide 1.5/268-281 was mapped near the top of the VSG. CONCLUSIONS: A biotinylated peptide corresponding to AA 268-281 of VSG LiTat 1.5 may replace the native VSG in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native VSG LiTat 1.3 and VSG LiTat 1.5. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Sleeping sickness en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Trypanosoma brucei gambiense en_US
dc.subject Vectors en_US
dc.subject Tsetse flies en_US
dc.subject Glossina morsitans en_US
dc.subject Diagnosis en_US
dc.subject Peptides en_US
dc.subject Variable surface glycoproteins en_US
dc.subject VSG en_US
dc.subject LiTat 1.5 en_US
dc.subject Amino acid sequences en_US
dc.subject Mimotopes en_US
dc.subject Accuracy en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Laboratory techniques and procedures en_US
dc.title A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for Trypanosoma brucei gambiense en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 18 en_US
dc.citation.pages 461-465 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/23289521 en_US
dc.citation.jabbreviation Trop Med Int Health en_US


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