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Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins

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dc.contributor.author Victor, B. en_US
dc.contributor.author Dorny, P. en_US
dc.contributor.author Kanobana, K. en_US
dc.contributor.author Polman, K. en_US
dc.contributor.author Lindh, J. en_US
dc.contributor.author Deelder, A. M. en_US
dc.contributor.author Palmblad, M. en_US
dc.contributor.author Gabriël, S. en_US
dc.date.accessioned 2014-09-25T13:39:37Z
dc.date.available 2014-09-25T13:39:37Z
dc.date.issued 2013 en_US
dc.identifier.issn 1756-0500 en_US
dc.identifier.doi http://dx.doi.org/10.1186/1756-0500-6-224 en_US
dc.identifier.other ITG-B1A; ITG-B2A; ITG-B3B; ITG-B4A; ITG-BLA; DBM; U-MHELM; U-VHELM; DOI; FTA; E-only; Abstract; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/7768
dc.description.abstract BACKGROUND: Taenia solium taeniasis/cysticercosis is a zoonotic helminth infection mainly found in rural regions of Africa, Asia and Latin America. In endemic areas, diagnosis of cysticercosis largely depends on serology, but these methods have their drawbacks and require improvement. This implies better knowledge of the proteins secreted and excreted by the parasite. In a previous study, we used a custom protein database containing protein sequences from related helminths to identify T. solium metacestode excretion/secretion proteins. An alternative or complementary approach would be to use expressed sequence tags combined with BLAST and protein mapping to supercontigs of Echinococcus granulosus, a closely related cestode. In this study, we evaluate this approach and compare the results to those obtained in the previous study. FINDINGS: We report 297 proteins organized in 106 protein groups based on homology. Additional classification was done using Gene Ontology information on biological process and molecular function. Of the 106 protein groups, 58 groups were newly identified, while 48 groups confirmed previous findings. Blast2GO analysis revealed that the majority of the proteins were involved in catalytic activities and binding. CONCLUSIONS: In this study, we used translated expressed sequence tags combined with BLAST and mapping strategies to both confirm and complement previous research. Our findings are comparable to recent studies on other helminth genera like Echinococcus, Schistosoma and Clonorchis, indicating similarities between helminth excretion/secretion proteomes. en_US
dc.language English en_US
dc.subject Helminthic diseases en_US
dc.subject Zoonoses en_US
dc.subject Neurocysticercosis en_US
dc.subject Taenia solium en_US
dc.subject Metacestodes en_US
dc.subject Proteomics en_US
dc.subject Identification en_US
dc.subject Proteins en_US
dc.subject Expression en_US
dc.subject Sequencing en_US
dc.subject Classification en_US
dc.subject Peru en_US
dc.subject America, Latin en_US
dc.subject Zambia en_US
dc.subject Africa, Southern en_US
dc.title Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins en_US
dc.type Article-E en_US
dc.citation.issue 224 en_US
dc.citation.jtitle BMC Research Notes en_US
dc.citation.volume 6 en_US
dc.citation.pages 1-8 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/23742691 en_US
dc.identifier.url http://www.biomedcentral.com/1756-0500/6/224 en_US
dc.citation.jabbreviation BMC Res Notes en_US


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