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A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood

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dc.contributor.author Fikru, R. en_US
dc.contributor.author Hagos, A. en_US
dc.contributor.author Rogé, S. en_US
dc.contributor.author Reyna-Bello, A. en_US
dc.contributor.author Gonzatti, M. I. en_US
dc.contributor.author Merga, B. en_US
dc.contributor.author Goddeeris, B. M. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2014-09-25T13:39:47Z
dc.date.available 2014-09-25T13:39:47Z
dc.date.issued 2014 en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0084819 en_US
dc.identifier.other ITG-B1B; ITG-B3B; ITG-BLA; DBM; U-PARDIA; JIF; DOI; FTA; E-only; OAJ; Abstract; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/7876
dc.description.abstract A study was conducted to develop a Trypanosoma vivax (T. vivax) specific PCR based on the T. vivax proline racemase (TvPRAC) gene. Forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR was conducted on DNA extracted from different haemotropic pathogens: T. vivax from Nigeria, Ethiopia and Venezuela, T. congolense Savannah type, T. brucei brucei, T. evansi, T. equiperdum, T. theileri, Theileria parva, Anaplasma marginale, Babesia bovis and Babesia bigemina and from bovine, goat, mouse, camel and human blood. The analytical sensitivity of the TvPRAC PCR was compared with that of the ITS-1 PCR and the 18S PCR-RFLP on a dilution series of T. vivax DNA in water. The diagnostic performance of the three PCRs was compared on 411 Ethiopian bovine blood specimens collected in a former study. TvPRAC PCR proved to be fully specific for T. vivax, irrespective of its geographical origin. Its analytical sensitivity was lower than that of ITS-1 PCR. On these bovine specimens, TvPRAC PCR detected 8.3% T. vivax infections while ITS-1 PCR and 18S PCR-RFLP detected respectively 22.6 and 6.1% T. vivax infections. The study demonstrates that a proline racemase based PCR could be used, preferably in combination with ITS-1 PCR, as a species-specific diagnostic test for T. vivax infections worldwide. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Animal diseases en_US
dc.subject Nagana en_US
dc.subject Trypanosoma vivax en_US
dc.subject Vectors en_US
dc.subject Tsetse flies en_US
dc.subject Glossina morsitans en_US
dc.subject Cattle en_US
dc.subject Identification en_US
dc.subject Blood en_US
dc.subject Serology en_US
dc.subject Polymerase chain reaction en_US
dc.subject PCR en_US
dc.subject Evaluation en_US
dc.subject Performance en_US
dc.subject Accuracy en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Laboratory techniques and procedures en_US
dc.title A proline racemase based PCR for identification of Trypanosoma vivax in cattle blood en_US
dc.type Article-E en_US
dc.citation.issue 1 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.volume 9 en_US
dc.citation.pages e84819 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/24416292 en_US
dc.citation.jabbreviation PLoS ONE en_US


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