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Circulating CD14brightCD16+ 'intermediate' monocytes exhibit enhanced parasite pattern recognition in human helminth infection

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dc.contributor.author Turner, J. D. en_US
dc.contributor.author Bourke, C. D. en_US
dc.contributor.author Meurs, L. en_US
dc.contributor.author Mbow, M. en_US
dc.contributor.author Dieye, T. N. en_US
dc.contributor.author Mboup, S. en_US
dc.contributor.author Polman, K. en_US
dc.contributor.author Mountford, A. P. en_US
dc.date.accessioned 2014-09-25T13:40:00Z
dc.date.available 2014-09-25T13:40:00Z
dc.date.issued 2014 en_US
dc.identifier.issn 1935-2727 en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0002817 en_US
dc.identifier.other ITG-B3B; ITG-B4B; ITG-B7A; DBM; U-MHELM; JIF; DOI; FTA; OAJ; Abstract; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/8037
dc.description.abstract Circulating monocyte sub-sets have recently emerged as mediators of divergent immune functions during infectious disease but their role in helminth infection has not been investigated. In this study we evaluated whether 'classical' (CD14brightCD16-), 'intermediate' (CD14brightCD16+), and 'non-classical' (CD14dimCD16+) monocyte sub-sets from peripheral blood mononuclear cells varied in both abundance and ability to bind antigenic material amongst individuals living in a region of Northern Senegal which is co-endemic for Schistosoma mansoni and S. haematobium. Monocyte recognition of excretory/secretory (E/S) products released by skin-invasive cercariae, or eggs, of S. mansoni was assessed by flow cytometry and compared between S. mansoni mono-infected, S. mansoni and S. haematobium co-infected, and uninfected participants. Each of the three monocyte sub-sets in the different infection groups bound schistosome E/S material. However, 'intermediate' CD14brightCD16+ monocytes had a significantly enhanced ability to bind cercarial and egg E/S. Moreover, this elevation of ligand binding was particularly evident in co-infected participants. This is the first demonstration of modulated parasite pattern recognition in CD14brightCD16+ intermediate monocytes during helminth infection, which may have functional consequences for the ability of infected individuals to respond immunologically to infection. en_US
dc.language English en_US
dc.subject Helminthic diseases en_US
dc.subject Schistosomiasis en_US
dc.subject Schistosoma mansoni en_US
dc.subject Schistosoma haematobium en_US
dc.subject Immune activation en_US
dc.subject Antigens, CD14 en_US
dc.subject Antigens, CD16 en_US
dc.subject Monocytes en_US
dc.subject Peripheral blood mononuclear cell (PBMC) en_US
dc.subject PBMC assay en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Senegal en_US
dc.subject Africa, West en_US
dc.title Circulating CD14brightCD16+ 'intermediate' monocytes exhibit enhanced parasite pattern recognition in human helminth infection en_US
dc.type Article-E en_US
dc.citation.issue 4 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.volume 8 en_US
dc.citation.pages e2817 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/24762736 en_US
dc.citation.jabbreviation PLoS Negl Trop Dis en_US


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