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Insertion sequence element single nucleotide polymorphism typing provides insights into the population structure and evolution of Mycobacterium ulcerans across Africa

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Show simple item record Vandelannoote, K. en_US Jordaens, K. en_US Bomans, P. en_US Leirs, H. en_US Durnez, L. en_US Affolabi, D. en_US Sopoh, G. en_US Aguiar, J. en_US Phanzu, D. M. en_US Kibadi, K. en_US Eyangoh, S. en_US Manou, L. B. en_US Phillips, R. O. en_US Adjei, O. en_US Ablordey, A. en_US Rigouts, L. en_US Portaels, F. en_US Eddyani, M. en_US de Jong, B. C. en_US 2014-09-25T13:40:03Z 2014-09-25T13:40:03Z 2014 en_US
dc.identifier.issn 0099-2240 en_US
dc.identifier.doi en_US
dc.identifier.other ITG-B1B; ITG-B3B; ITG-B5B; ITG-B16A; ITG-X17B; ITG-B18B; ITG-BLA; DBM; U-MYCOB; JIF; DOI; PDF; Abstract, DSPACE56 en_US
dc.description.abstract Buruli ulcer is an indolent, slowly progressing necrotizing disease of the skin caused by infection with Mycobacterium ulcerans. In the present study we applied a redesigned technique to a vast panel of M. ulcerans disease isolates and clinical samples originating from multiple African disease foci (i) to gain fundamental insights into the population structure and evolutionary history of the pathogen and (ii) to disentangle the phylogeographic relationships within the genetically conserved cluster of African M. ulcerans. Our analyses identified 23 different African insertion sequence element single nucleotide polymorphism (ISE-SNP) types which dominate in different Buruli ulcer endemic areas. These ISE-SNP types appear to be the initial stages of clonal diversification from a common, possibly ancestral, ISE-SNP type. ISE-SNP types were found unevenly distributed over the greater West African hydrological drainage basins. Our findings suggest that geographical barriers bordering the basins to some extent prevented bacterial gene flow between basins and that this resulted in independent focal transmission clusters associated with the hydrological drainage areas. Different phylogenetic methods yielded two well supported sister clades within the African ISE-SNP types. The ISE-SNP types from the "pan-African clade" were found widespread throughout Africa while the ISE-SNP types of the "Gabonese/Cameroonian clade" were much rarer and found in a more restricted area, which suggested that the latter clade evolved more recently. Additionally, the Gabonese/Cameroonian clade was found to form a strongly supported monophyletic group with Papua New Guinean ISE-SNP type 8, which was unrelated to other Southeast Asian ISE-SNP types. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Buruli ulcer en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Evolutionary genetics en_US
dc.subject Population structure en_US
dc.subject Cluster analysis en_US
dc.subject Pathogenesis en_US
dc.subject Phylogeography en_US
dc.subject Nucleotides en_US
dc.subject Polymorphism en_US
dc.subject Sequence variation en_US
dc.subject Genetic diversity en_US
dc.subject Africa, General en_US
dc.title Insertion sequence element single nucleotide polymorphism typing provides insights into the population structure and evolution of Mycobacterium ulcerans across Africa en_US
dc.type Article en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Applied and Environmental Microbiology en_US
dc.citation.volume 80 en_US
dc.citation.pages 1197-1209 en_US
dc.identifier.pmid en_US
dc.citation.jabbreviation Appl Environ Microbiol en_US

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