The need for transparency and good practices in the qPCR literature
Bustin, S. A.; Benes, V.; Garson, J.; Hellemans, J.; Huggett, J.; Kubista, M.; Mueller, R.; Nolan, T.; Pfaffl, M. W.; Shipley, G.; Wittwer, C. T.; Schjerling, P.; Day, P. J.; Abreu, M.; Aguado, B.; Beaulieu, J. F.; Beckers, A.; Bogaert, S.; Browne, J. A.; Carrasco-Ramiro, F.; Ceelen, L.; Ciborowski, K.; Cornillie, P.; Coulon, S.; Cuypers, A.; De Brouwer, S.; De Ceuninck, L.; De Craene, J.; De Naeyer, H.; De Spiegelaere, W.; Deckers, K.; Dheedene, A.; Durinck, K.; Ferreira-Teixeira, M.; Fieuw, A.; Gallup, J. M.; Gonzalo-Flores, S.; Goossens, K.; Heindryckx, F.; Herring, E.; Hoenicka, H.; Icardi, L.; Jaggi, R.; Javad, F.; Karampelias, M.; Kibenge, F.; Kibenge, M.; Kumps, C.; Lambertz, I.; Lammens, T.; Markey, A.; Messiaen, P.; Mets, E.; Morais, S.; Mudarra-Rubio, A.; Nakiwala, J.; Nelis, H.; Olsvik, P. A.; Perez-Novo, C.; Plusquin, M.; Remans, T.; Rihani, A.; Rodrigues-Santos, P.; Rondou, P.; Sanders, R.; Schmidt-Bleek, K.; Skovgaard, K.; Smeets, K.; Tabera, L.; Toegel, S.; Van Acker, T.; Van den Broeck, W.; Van der Meulen, J.; Van Gele, M.; Van Peer, G.; Van Poucke, M.; Van Roy, N.; Vergult, S.; Wauman, J.; Tshuikina-Wiklander, M.; Willems, E.; Zaccara, S.; Zeka, F.; Vandesompele, J.
Journal Name:
Nature Methods
Keywords:
Detection
Polymerase chain reaction
PCR
Information
Quantitative research
Guideline adherence
Journal impact factors
Scientific literature
Review of the literature
Abstract:
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
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