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The need for transparency and good practices in the qPCR literature

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dc.contributor.author Bustin, S. A. en_US
dc.contributor.author Benes, V. en_US
dc.contributor.author Garson, J. en_US
dc.contributor.author Hellemans, J. en_US
dc.contributor.author Huggett, J. en_US
dc.contributor.author Kubista, M. en_US
dc.contributor.author Mueller, R. en_US
dc.contributor.author Nolan, T. en_US
dc.contributor.author Pfaffl, M. W. en_US
dc.contributor.author Shipley, G. en_US
dc.contributor.author Wittwer, C. T. en_US
dc.contributor.author Schjerling, P. en_US
dc.contributor.author Day, P. J. en_US
dc.contributor.author Abreu, M. en_US
dc.contributor.author Aguado, B. en_US
dc.contributor.author Beaulieu, J. F. en_US
dc.contributor.author Beckers, A. en_US
dc.contributor.author Bogaert, S. en_US
dc.contributor.author Browne, J. A. en_US
dc.contributor.author Carrasco-Ramiro, F. en_US
dc.contributor.author Ceelen, L. en_US
dc.contributor.author Ciborowski, K. en_US
dc.contributor.author Cornillie, P. en_US
dc.contributor.author Coulon, S. en_US
dc.contributor.author Cuypers, A. en_US
dc.contributor.author De Brouwer, S. en_US
dc.contributor.author De Ceuninck, L. en_US
dc.contributor.author De Craene, J. en_US
dc.contributor.author De Naeyer, H. en_US
dc.contributor.author De Spiegelaere, W. en_US
dc.contributor.author Deckers, K. en_US
dc.contributor.author Dheedene, A. en_US
dc.contributor.author Durinck, K. en_US
dc.contributor.author Ferreira-Teixeira, M. en_US
dc.contributor.author Fieuw, A. en_US
dc.contributor.author Gallup, J. M. en_US
dc.contributor.author Gonzalo-Flores, S. en_US
dc.contributor.author Goossens, K. en_US
dc.contributor.author Heindryckx, F. en_US
dc.contributor.author Herring, E. en_US
dc.contributor.author Hoenicka, H. en_US
dc.contributor.author Icardi, L. en_US
dc.contributor.author Jaggi, R. en_US
dc.contributor.author Javad, F. en_US
dc.contributor.author Karampelias, M. en_US
dc.contributor.author Kibenge, F. en_US
dc.contributor.author Kibenge, M. en_US
dc.contributor.author Kumps, C. en_US
dc.contributor.author Lambertz, I. en_US
dc.contributor.author Lammens, T. en_US
dc.contributor.author Markey, A. en_US
dc.contributor.author Messiaen, P. en_US
dc.contributor.author Mets, E. en_US
dc.contributor.author Morais, S. en_US
dc.contributor.author Mudarra-Rubio, A. en_US
dc.contributor.author Nakiwala, J. en_US
dc.contributor.author Nelis, H. en_US
dc.contributor.author Olsvik, P. A. en_US
dc.contributor.author Perez-Novo, C. en_US
dc.contributor.author Plusquin, M. en_US
dc.contributor.author Remans, T. en_US
dc.contributor.author Rihani, A. en_US
dc.contributor.author Rodrigues-Santos, P. en_US
dc.contributor.author Rondou, P. en_US
dc.contributor.author Sanders, R. en_US
dc.contributor.author Schmidt-Bleek, K. en_US
dc.contributor.author Skovgaard, K. en_US
dc.contributor.author Smeets, K. en_US
dc.contributor.author Tabera, L. en_US
dc.contributor.author Toegel, S. en_US
dc.contributor.author Van Acker, T. en_US
dc.contributor.author Van den Broeck, W. en_US
dc.contributor.author Van der Meulen, J. en_US
dc.contributor.author Van Gele, M. en_US
dc.contributor.author Van Peer, G. en_US
dc.contributor.author Van Poucke, M. en_US
dc.contributor.author Van Roy, N. en_US
dc.contributor.author Vergult, S. en_US
dc.contributor.author Wauman, J. en_US
dc.contributor.author Tshuikina-Wiklander, M. en_US
dc.contributor.author Willems, E. en_US
dc.contributor.author Zaccara, S. en_US
dc.contributor.author Zeka, F. en_US
dc.contributor.author Vandesompele, J. en_US
dc.date.accessioned 2014-09-25T13:40:06Z
dc.date.available 2014-09-25T13:40:06Z
dc.date.issued 2013 en_US
dc.identifier.issn 1548-7091 en_US
dc.identifier.doi http://dx.doi.org/10.1038/nmeth.2697 en_US
dc.identifier.other ITG-B56B; DBM; U-IMMUN; JIF; DOI; Not available; UPD56 en_US
dc.identifier.uri http://hdl.handle.net/10390/8105
dc.description.abstract Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not. en_US
dc.language English en_US
dc.subject Detection en_US
dc.subject Polymerase chain reaction en_US
dc.subject PCR en_US
dc.subject Information en_US
dc.subject Quantitative research en_US
dc.subject Guideline adherence en_US
dc.subject Journal impact factors en_US
dc.subject Scientific literature en_US
dc.subject Review of the literature en_US
dc.title The need for transparency and good practices in the qPCR literature en_US
dc.type Article en_US
dc.citation.issue 11 en_US
dc.citation.jtitle Nature Methods en_US
dc.citation.volume 10 en_US
dc.citation.pages 1063-1067 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/24173381 en_US
dc.citation.jabbreviation Nat Methods en_US


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