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Delivery of a functional anti-trypanosome nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius

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Show simple item record De Vooght, L. Caljon, G. De Ridder, K. Van Den Abbeele, J. 2015-01-16T13:30:27Z 2015-01-16T13:30:27Z 2014
dc.identifier.issn 1475-2859
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B2B
dc.identifier.other ITG-B3B
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-VPROT
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other URL
dc.identifier.other CPDF
dc.identifier.other OAJ
dc.identifier.other E-only
dc.identifier.other Abstract
dc.identifier.other UPD57
dc.description.abstract Background: Sodalis glossinidius, a vertically transmitted microbial symbiont of the tsetse fly, is currently considered as a potential delivery system for anti-trypanosomal components that reduce or eliminate the capability of the tsetse fly host to transmit parasitic trypanosomes, an approach also known as paratransgenesis. An essential step in developing paratransgenic tsetse is the stable colonization of adult flies and their progeny with recombinant Sodalis bacteria, expressing trypanocidal effector molecules in tissues where the parasite resides. Results: In this study, Sodalis was tested for its ability to deliver functional anti-trypanosome nanobodies (Nbs) in Glossina morsitans morsitans. We characterized the in vitro and in vivo stability of recombinant Sodalis (recSodalis) expressing a potent trypanolytic nanobody, i.e. Nb_An46. We show that recSodalis is competitive with WT Sodalis in in vivo conditions and that tsetse flies transiently cleared of their endogenous WT Sodalis population can be successfully repopulated with recSodalis at high densities. In addition, vertical transmission to the offspring was observed. Finally, we demonstrated that recSodalis expressed significant levels (ng range) of functional Nb_An46 in different tsetse fly tissues, including the midgut where an important developmental stage of the trypanosome parasite occurs. Conclusions: We demonstrated the proof-of-concept that the Sodalis symbiont can be genetically engineered to express and release significant amounts of functional anti-trypanosome Nbs in different tissues of the tsetse fly. The application of this innovative concept of using pathogen-targeting nanobodies delivered by insect symbiotic bacteria could be extended to other vector-pathogen systems. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, African en_US
dc.subject Sleeping sickness en_US
dc.subject Vectors en_US
dc.subject Tsetse flies en_US
dc.subject Glossina morsitans morsitans en_US
dc.subject Symbionts en_US
dc.subject Bacteria en_US
dc.subject Sodalis glossinidius en_US
dc.subject Nanobodies en_US
dc.subject Characterization en_US
dc.subject Recombinants en_US
dc.subject In vivo en_US
dc.subject In vitro en_US
dc.subject Disease transmission, vertical en_US
dc.subject Genetic manipulation en_US
dc.title Delivery of a functional anti-trypanosome nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius en_US
dc.type Article-E en_US
dc.citation.issue 156 en_US
dc.citation.jtitle Microbial Cell Factories en_US
dc.citation.volume 13 en_US
dc.citation.jabbreviation Microb Cell Fact en_US

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