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Direct Leishmania species typing in Old World clinical samples: evaluation of 3 sensitive methods based on the heat-shock protein 70 gene

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dc.contributor.author Montalvo, A. M.
dc.contributor.author Fraga, J.
dc.contributor.author El Safi, S.
dc.contributor.author Gramiccia, M.
dc.contributor.author Jaffe, C. L.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Van der Auwera, G.
dc.date.accessioned 2015-02-05T14:46:49Z
dc.date.available 2015-02-05T14:46:49Z
dc.date.issued 2014
dc.identifier.issn 0732-8893
dc.identifier.doi http://dx.doi.org/10.1016/j.diagmicrobio.2014.05.012
dc.identifier.other ITG-B6A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-MOLPAR
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD57
dc.identifier.uri http://hdl.handle.net/10390/8254
dc.description.abstract In the diagnosis of leishmaniasis, identification of the causative Leishmania species is relevant for treatment, prognosis, and epidemiology. Three new hsp70-based PCR variants were developed and recently validated on clinical samples from Peru, without the need for culturing. We evaluated their performance on 133 clinical samples (bone marrow, blood, buffy coat, lymph node aspirates, lesion biopsies) from 42 cutaneous and 56 visceral leishmaniasis patients and 35 negative cases, all from Old World countries (Italy, Sudan, Israel, and Tunisia). The 3 new PCRs were significantly more sensitive than those previously described for hsp70, and their respective restriction fragment analyses were more efficient for species identification. In 79% of the parasitologically confirmed positive samples, the species could be identified directly from sample DNA. This evaluation demonstrated that these new tools are globally applicable in different geographical, clinical, and sampling contexts, and they could become the reference method for identification of Leishmania species in clinical specimens. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Kala azar en_US
dc.subject Cutaneous en_US
dc.subject Visceral en_US
dc.subject Leishmaniasis en_US
dc.subject Leishmania en_US
dc.subject Evaluation en_US
dc.subject Performance en_US
dc.subject Sampling en_US
dc.subject Bone marrow en_US
dc.subject Blood en_US
dc.subject Buffy coat en_US
dc.subject Lymph nodes en_US
dc.subject Biopsy en_US
dc.subject Identification en_US
dc.subject hsp70 en_US
dc.subject Variants en_US
dc.subject Polymerase chain reaction en_US
dc.subject PCR en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Peru en_US
dc.subject America, Latin en_US
dc.title Direct Leishmania species typing in Old World clinical samples: evaluation of 3 sensitive methods based on the heat-shock protein 70 gene en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Diagnostic Microbiology and Infectious Disease en_US
dc.citation.volume 80 en_US
dc.citation.pages 35-39 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/25038029
dc.citation.jabbreviation Diagn Microbiol Infect Dis en_US


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