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Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture

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dc.contributor.author Noulin, F.
dc.contributor.author Manesia, J. K.
dc.contributor.author Rosanas-Urgell, A.
dc.contributor.author Erhart, A.
dc.contributor.author Borlon, C.
dc.contributor.author Van Den Abbeele, J.
dc.contributor.author D'Alessandro, U.
dc.contributor.author Verfaillie, C. M.
dc.date.accessioned 2015-02-10T15:18:56Z
dc.date.available 2015-02-10T15:18:56Z
dc.date.issued 2014
dc.identifier.issn 1932-6203
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0112496
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B3A
dc.identifier.other ITG-B4A
dc.identifier.other ITG-B5B
dc.identifier.other ITG-B6A
dc.identifier.other ITG-N7H
dc.identifier.other DBM
dc.identifier.other U-MALAR
dc.identifier.other U-VPROT
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other OAJ
dc.identifier.other E-only
dc.identifier.other Abstract
dc.identifier.other UPD57
dc.identifier.uri http://hdl.handle.net/10390/8262
dc.description.abstract The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Malaria en_US
dc.subject Plasmodium vivax en_US
dc.subject Vectors en_US
dc.subject Mosquitoes en_US
dc.subject Anopheles en_US
dc.subject In vitro en_US
dc.subject Culture en_US
dc.subject Reticulocytes en_US
dc.subject Red blood cells en_US
dc.subject Hematopoietic stem cells en_US
dc.subject Peripheral blood en_US
dc.subject Bone marrow en_US
dc.subject CD34+ en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture en_US
dc.type Article-E en_US
dc.citation.issue 11 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.volume 9 en_US
dc.citation.pages e112496 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/25393299
dc.citation.jabbreviation PLoS ONE en_US


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