Institute of Tropical Medicine Antwerp
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Development of a latex agglutination test with recombinant variant surface glycoprotein for serodiagnosis of surra

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dc.contributor.author Rogé, S.
dc.contributor.author Baelmans, R.
dc.contributor.author Claes, F.
dc.contributor.author Lejon, V.
dc.contributor.author Guisez, Y.
dc.contributor.author Jacquet, D.
dc.contributor.author Büscher, P.
dc.date.accessioned 2015-02-17T10:26:03Z
dc.date.available 2015-02-17T10:26:03Z
dc.date.issued 2014
dc.identifier.issn 0304-4017
dc.identifier.doi http://dx.doi.org/10.1016/j.vetpar.2014.08.026
dc.identifier.other ITG-B1B
dc.identifier.other ITG-I2B
dc.identifier.other ITG-I6B
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-PARDIA
dc.identifier.other INTER
dc.identifier.other U-TTP
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD57
dc.identifier.uri http://hdl.handle.net/10390/8280
dc.description.abstract Serodiagnosis of surra is commonly performed with the CATT/Trypanosoma evansi direct agglutination test. This antibody detection test is based on lyophilised bloodstream form trypanosomes propagated in rats and presenting the predominant variant surface glycoprotein (VSG) RoTat 1.2 on their surface. Recently, the N-terminal fragment of VSG RoTat 1.2 has been expressed as a recombinant protein in the yeast Pichia pastoris and showed diagnostic potential in ELISA. This recombinant antigen has now been incorporated in a latex agglutination test, the rLATEX/T. evansi. In this study, we compared the diagnostic accuracy of rLATEX/T. evansi and CATT/T. evansi with immune trypanolysis (TL) as reference test on a total of 1717 sera from camels, horses, bovines, water buffaloes, dogs and sheep. The rLATEX/T. evansi displayed a slightly better agreement with TL than CATT/T. evansi (kappa [kappa] respectively 0.84 and 0.72). The sensitivities of rLATEX/T. evansi (84.2%, 95% CI 80.8-87.1) and CATT/T. evansi (84.0%, 95% CI 80.6-87.0) were similar, but rLATEX/T. evansi was significantly more specific (97.7%, 95% CI 96.7-98.4) than CATT/T. evansi (89.4%; 95% CI 87.6-91.1). We consider the rLATEX/T. evansi an alternative for the CATT/T. evansi, with the advantage that the use of a purified recombinant antigen leads to a more standardised diagnostic test with an improved specificity. Moreover, it eliminates the use of laboratory animals and can be easily scaled-up, e.g. in biofermentors. en_US
dc.language English en_US
dc.subject Animal diseases en_US
dc.subject Surra en_US
dc.subject Trypanosoma evansi en_US
dc.subject Camels en_US
dc.subject Horses en_US
dc.subject Bovines en_US
dc.subject Dogs en_US
dc.subject Sheep en_US
dc.subject Diagnosis en_US
dc.subject Latex agglutination test en_US
dc.subject Evaluation en_US
dc.subject Performance en_US
dc.subject Accuracy en_US
dc.subject Antigens en_US
dc.subject Trypanolysis en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Recombinant proteins en_US
dc.subject Yeasts en_US
dc.subject Pichia pastoris en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Development of a latex agglutination test with recombinant variant surface glycoprotein for serodiagnosis of surra en_US
dc.type Article en_US
dc.citation.issue 3-4 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 205 en_US
dc.citation.pages 460-465 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/25224790
dc.citation.jabbreviation Vet Parasitol en_US


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