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Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers

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Show simple item record Brisse, S. en_US Dujardin, J. C. en_US Tibayrenc, M. en_US 2007-12-06T14:33:13Z 2007-12-06T14:33:13Z 2000 en_US
dc.identifier.issn 0166-6851 en_US
dc.identifier.other ITG-P2A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract Six discrete phylogenetic lineages were recently identified in Trypanosoma cruzi, on the basis of multilocus enzyme electrophoresis and random amplified polymorphic DNA (RAPD) characterisation. The objective of the present study was to develop specific PCR-based markers for the identification of each of the six lineages. Eighty-seven T. cruzi stocks representative of all the lineages were characterised by RAPD with three primers, resulting in the identification of three fragments that were specifically amplified in the given sets of lineages. After cloning and sequencing these fragments, three pairs of sequence-characterised amplified region (SCAR) primers were designed. After PCR amplification using the SCAR primers, the initial polymorphism was retained either as the presence or absence of amplification, or as size variation between the PCR products. Although most PCR products, taken individually, were distributed across several lineages, the combination of the three SCAR markers resulted in characteristic patterns that were distinct in the six lineages. Furthermore, T. cruzi lineages were distinguished from Trypanosoma rangeli, T. cruzi marinkellei and T. cruzi-like organisms. The excellent correspondence of these new PCR markers with the phylogenetic lineages, allied with their sensitivity, makes them reliable tools for lineage identification and strain characterisation in T. cruzi. The approach described here could be generalised to any species of microorganism harbouring clear-cut phylogenetic subdivisions. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoology en_US
dc.subject Trypanosoma cruzi en_US
dc.subject Identification en_US
dc.subject Characterization en_US
dc.subject Genetic variation en_US
dc.subject Sequence-characterised amplified regions (SCAR) en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis, American en_US
dc.subject Chagas disease en_US
dc.subject Epidemiology en_US
dc.title Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Molecular and Biochemical Parasitology en_US
dc.citation.volume 111 en_US
dc.citation.pages 95-105 en_US Amsterdam
dc.citation.jabbreviation Mol Biochem Parasitol en_US

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