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Microbial shifts in the aging mouse gut

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dc.contributor.author Langille, M. G.
dc.contributor.author Meehan, C. J.
dc.contributor.author Koenig, J. E.
dc.contributor.author Dhanani, A. S.
dc.contributor.author Rose, R. A.
dc.contributor.author Howlett, S. E.
dc.contributor.author Beiko, R. G.
dc.date.accessioned 2015-08-12T08:05:12Z
dc.date.available 2015-08-12T08:05:12Z
dc.date.issued 2014
dc.identifier.issn 2049-2618
dc.identifier.doi http://dx.doi.org/10.1186/s40168-014-0050-9
dc.identifier.other http://www.ncbi.nlm.nih.gov/pubmed/25520805
dc.identifier.other ITG-B2B
dc.identifier.other DBM
dc.identifier.other U-MYCOB
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other OAJ
dc.identifier.other E-only
dc.identifier.other Abstract
dc.identifier.other UPD58
dc.identifier.uri http://hdl.handle.net/10390/8368
dc.description.abstract BACKGROUND: The changes that occur in the microbiome of aging individuals are unclear, especially in light of the imperfect correlation of frailty with age. Studies in older human subjects have reported subtle effects, but these results may be confounded by other variables that often change with age such as diet and place of residence. To test these associations in a more controlled model system, we examined the relationship between age, frailty, and the gut microbiome of female C57BL/6 J mice. RESULTS: The frailty index, which is based on the evaluation of 31 clinical signs of deterioration in mice, showed a near-perfect correlation with age. We observed a statistically significant relationship between age and the taxonomic composition of the corresponding microbiome. Consistent with previous human studies, the Rikenellaceae family, which includes the Alistipes genus, was the most significantly overrepresented taxon within middle-aged and older mice. The functional profile of the mouse gut microbiome also varied with host age and frailty. Bacterial-encoded functions that were underrepresented in older mice included cobalamin (B12) and biotin (B7) biosynthesis, and bacterial SOS genes associated with DNA repair. Conversely, creatine degradation, associated with muscle wasting, was overrepresented within the gut microbiomes of the older mice, as were bacterial-encoded beta-glucuronidases, which can influence drug-induced epithelial cell toxicity. Older mice also showed an overabundance of monosaccharide utilization genes relative to di-, oligo-, and polysaccharide utilization genes, which may have a substantial impact on gut homeostasis. CONCLUSION: We have identified taxonomic and functional patterns that correlate with age and frailty in the mouse microbiome. Differences in functions related to host nutrition and drug pharmacology vary in an age-dependent manner, suggesting that the availability and timing of essential functions may differ significantly with age and frailty. Future work with larger cohorts of mice will aim to separate the effects of age and frailty, and other factors. en_US
dc.language English en_US
dc.subject Microbiome en_US
dc.subject Aging en_US
dc.subject Mice en_US
dc.subject B-12 en_US
dc.subject Clinical manifestations en_US
dc.subject Signs and symptoms en_US
dc.title Microbial shifts in the aging mouse gut en_US
dc.type Article-E en_US
dc.citation.issue 50 en_US
dc.citation.jtitle Microbiome en_US
dc.citation.volume 2 en_US
dc.citation.pages 1-12 en_US
dc.citation.jabbreviation Microbiome en_US


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