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Whole genome comparisons suggest random distribution of Mycobacterium ulcerans genotypes in a Buruli ulcer endemic region of Ghana

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dc.contributor.author Ablordey, A. S.
dc.contributor.author Vandelannoote, K.
dc.contributor.author Frimpong, I. A.
dc.contributor.author Ahortor, E. K.
dc.contributor.author Amissah, N. A.
dc.contributor.author Eddyani, M.
dc.contributor.author Durnez, L.
dc.contributor.author Portaels, F.
dc.contributor.author de Jong, B. C.
dc.contributor.author Leirs, H.
dc.contributor.author Porter, J. L.
dc.contributor.author Mangas, K. M.
dc.contributor.author Lam, M. M.
dc.contributor.author Buultjens, A.
dc.contributor.author Seemann, T.
dc.contributor.author Tobias, N. J.
dc.contributor.author Stinear, T. P.
dc.date.accessioned 2015-08-20T14:24:10Z
dc.date.available 2015-08-20T14:24:10Z
dc.date.issued 2015
dc.identifier.issn 1935-2727
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0003681
dc.identifier.other ITG-B2B
dc.identifier.other ITG-B6B
dc.identifier.other ITG-B7B
dc.identifier.other ITG-X8B
dc.identifier.other ITG-B9A
dc.identifier.other DBM
dc.identifier.other U-MYCOB
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other OAJ
dc.identifier.other UPD59
dc.identifier.uri http://hdl.handle.net/10390/8425
dc.description.abstract Efforts to control the spread of Buruli ulcer - an emerging ulcerative skin infection caused by Mycobacterium ulcerans - have been hampered by our poor understanding of reservoirs and transmission. To help address this issue, we compared whole genomes from 18 clinical M. ulcerans isolates from a 30km2 region within the Asante Akim North District, Ashanti region, Ghana, with 15 other M. ulcerans isolates from elsewhere in Ghana and the surrounding countries of Ivory Coast, Togo, Benin and Nigeria. Contrary to our expectations of finding minor DNA sequence variations among isolates representing a single M. ulcerans circulating genotype, we found instead two distinct genotypes. One genotype was closely related to isolates from neighbouring regions of Amansie West and Densu, consistent with the predicted local endemic clone, but the second genotype (separated by 138 single nucleotide polymorphisms [SNPs] from other Ghanaian strains) most closely matched M. ulcerans from Nigeria, suggesting another introduction of M. ulcerans to Ghana, perhaps from that country. Both the exotic genotype and the local Ghanaian genotype displayed highly restricted intra-strain genetic variation, with less than 50 SNP differences across a 5.2Mbp core genome within each genotype. Interestingly, there was no discernible spatial clustering of genotypes at the local village scale. Interviews revealed no obvious epidemiological links among BU patients who had been infected with identical M. ulcerans genotypes but lived in geographically separate villages. We conclude that M. ulcerans is spread widely across the region, with multiple genotypes present in any one area. These data give us new perspectives on the behaviour of possible reservoirs and subsequent transmission mechanisms of M. ulcerans. These observations also show for the first time that M. ulcerans can be mobilized, introduced to a new area and then spread within a population. Potential reservoirs of M. ulcerans thus might include humans, or perhaps M. ulcerans-infected animals such as livestock that move regularly between countries. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Buruli ulcer en_US
dc.subject Mycobacterium ulcerans en_US
dc.subject Genome sequencing en_US
dc.subject Isolation en_US
dc.subject Genotypes en_US
dc.subject Genetic variation en_US
dc.subject Strains en_US
dc.subject Nucleotides en_US
dc.subject Polymorphism en_US
dc.subject Reservoirs en_US
dc.subject Imported diseases en_US
dc.subject Ghana en_US
dc.subject Togo en_US
dc.subject Benin en_US
dc.subject Nigeria en_US
dc.subject Africa, West en_US
dc.title Whole genome comparisons suggest random distribution of Mycobacterium ulcerans genotypes in a Buruli ulcer endemic region of Ghana en_US
dc.type Article-E en_US
dc.citation.issue 3 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.volume 9 en_US
dc.citation.pages e0003681 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/25826332
dc.citation.jabbreviation PLoS Negl Trop Dis en_US


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