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Evaluación de métodos fenotípicos para la detección de Staphylococcus aureus resistente a meticilina

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Show simple item record Horna, G. Astocondor, L. Jacobs, J. Garcia, C. 2015-08-28T08:44:42Z 2015-08-28T08:44:42Z 2015
dc.identifier.issn 0214-3429
dc.identifier.other ITG-C3A
dc.identifier.other DCS
dc.identifier.other U-TLM
dc.identifier.other JIF
dc.identifier.other UPD59
dc.description.abstract BACKGROUND: Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30mug) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS: Three hundred thirty-one strains of S. aureus isolated from blood cultures of patients from hospitals in Lima were used in the study. The following tests were performed: oxacillin screening agar (plates were inoculated with 4% NaCl and 6 mg/L of oxacillin), cefoxitin disc diffusion test (30 ug) and PCR to amplify the mecA gene. RESULTS: The mecA gene was detected in 165 out of 331 S. aureus isolates. Thus, the frequency of detection of MRSA was 50%. The evaluation of the cefoxitin disc diffusion test showed a 96.3% and 90.9% of sensitivity and specificity, respectively. CONCLUSION: Cefoxitin disc diffusion test correlated well with detection of the mecA gene by PCR. Therefore, this test can be an alternative to PCR for detection of MRSA in limited resources settings. en_US
dc.language Spanish en_US
dc.subject Bacterial diseases en_US
dc.subject Staphylococcal infections en_US
dc.subject Staphylococcus aureus en_US
dc.subject Comparison en_US
dc.subject Performance en_US
dc.subject Drug resistance en_US
dc.subject Methicillin en_US
dc.subject Detection en_US
dc.subject Screening en_US
dc.subject Polymerase chain reaction en_US
dc.subject PCR en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Evaluación de métodos fenotípicos para la detección de Staphylococcus aureus resistente a meticilina en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Revista Española de Quimioterapia en_US
dc.citation.volume 28 en_US
dc.citation.pages 98-100 en_US
dc.citation.jabbreviation Rev Esp Quimioter en_US

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