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A DNA tool for early detection of vaginal dysbiosis in African women

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dc.contributor.author Jespers, V.
dc.contributor.author Crucitti, T.
dc.contributor.author van de Wijgert, J.
dc.contributor.author Vaneechoutte, M.
dc.contributor.author Delany-Moretlwe, S.
dc.contributor.author Mwaura, M.
dc.contributor.author Agabe, S.
dc.contributor.author Menten, J.
dc.date.accessioned 2016-05-18T13:52:44Z
dc.date.available 2016-05-18T13:52:44Z
dc.date.issued 2016
dc.identifier.issn 0923-2508
dc.identifier.doi http://dx.doi.org/10.1016/j.resmic.2015.10.006
dc.identifier.other ITG-H1B
dc.identifier.other ITG-C2A
dc.identifier.other ITG- CLA
dc.identifier.other MULTI
dc.identifier.other DPH
dc.identifier.other U-ECHIV
dc.identifier.other DCS
dc.identifier.other U-ARLAB
dc.identifier.other U-CTU
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD61
dc.identifier.uri http://hdl.handle.net/10390/8814
dc.description.abstract A next-generation diagnostic tool for bacterial vaginosis, consisting of quantitative and/or qualitative molecular criteria, has not yet been identified. The optimal diagnostic tool should not only diagnose bacterial vaginosis in diverse populations, but should also detect early signs of transition to dysbiosis. We evaluated a tool based on log10-transformed qPCR data for Lactobacillus crispatus, L. iners, L. jensenii, L. gasseri, L. vaginalis, Lactobacillus genus, Atopobium vaginae and Gardnerella vaginalis in vaginal specimens of 426 African women to detect dysbiosis and predict transition to dysbiosis. G. vaginalis (p=0.204) and A. vaginae (p=0.001) were more commonly present in women who evolved to an intermediate (Nugent 4-6) or bacterial vaginosis score (Nugent 7-10) compared to women who continued to have a normal Nugent score. The combination of G. vaginalis, A. vaginae and Lactobacillus genus counts performed best for diagnostic accuracy for bacterial vaginosis--sensitivity 93.4% and specificity 83.6%; and for predictive accuracy for bacterial vaginosis--sensitivity 79% and specificity 52%. L. crispatus combinations did not perform well. We conclude that a triple-G. vaginalis-A. vaginae-Lactobacillus genus-qPCR tool holds promise for research in sub-Saharan Africa or when developed as a next-generation clinical diagnostic modality for bacterial vaginosis, ideally engineered as a rapid assay. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Vaginosis en_US
dc.subject Lactobacillus vaginalis en_US
dc.subject Lactobacillus crispatus en_US
dc.subject Gardnerella vaginalis en_US
dc.subject Dysbiosis en_US
dc.subject Women en_US
dc.subject Diagnosis en_US
dc.subject Rapid diagnostic tests en_US
dc.subject Accuracy en_US
dc.subject Specificity en_US
dc.subject Sensitivity en_US
dc.subject Polymerase chain reaction en_US
dc.subject PCR en_US
dc.subject Africa, General en_US
dc.title A DNA tool for early detection of vaginal dysbiosis in African women en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Research in Microbiology en_US
dc.citation.volume 167 en_US
dc.citation.pages 133-141 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/26577657
dc.citation.jabbreviation Res Microbiol en_US


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