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A Mycobacterial perspective on tuberculosis in West Africa: significant geographical variation of M. africanum and other M. tuberculosis complex lineages

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Show simple item record Gehre, F. Kumar, S. Kendall, L. Ejo, M. Secka, O. Ofori-Anyinam, B. Abatih, E. Antonio, M. Berkvens, D. de Jong, B. C. 2016-06-02T09:17:00Z 2016-06-02T09:17:00Z 2016
dc.identifier.issn 1935-2727
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B2B
dc.identifier.other ITG-B4B
dc.identifier.other ITG-B6B
dc.identifier.other ITG-B7B
dc.identifier.other ITG-B9A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-MYCOB
dc.identifier.other U-VEPID
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other OAJ
dc.identifier.other Abstract
dc.identifier.other UPD62
dc.description.abstract BACKGROUND: Phylogenetically distinct Mycobacterium tuberculosis lineages differ in their phenotypes and pathogenicity. Consequently, understanding mycobacterial population structures phylogeographically is essential for design, interpretation and generalizability of clinical trials. Comprehensive efforts are lacking to date to establish the West African mycobacterial population structure on a sub-continental scale, which has diagnostic implications and can inform the design of clinical TB trials. METHODOLOGY/PRINCIPAL FINDINGS: We collated novel and published genotyping (spoligotyping) data and classified spoligotypes into mycobacterial lineages/families using TBLineage and Spotclust, followed by phylogeographic analyses using statistics (logistic regression) and lineage axis plot analysis in GenGIS, in which a phylogenetic tree constructed in MIRU-VNTRplus was analysed. Combining spoligotyping data from 16 previously published studies with novel data from The Gambia, we obtained a total of 3580 isolates from 12 countries and identified 6 lineages comprising 32 families. By using stringent analytical tools we demonstrate for the first time a significant phylogeographic separation between western and eastern West Africa not only of the two M. africanum (West Africa 1 and 2) but also of several major M. tuberculosis sensu stricto families, such as LAM10 and Haarlem 3. Moreover, in a longitudinal logistic regression analysis for grouped data we showed that M. africanum West Africa 2 remains a persistent health concern. CONCLUSIONS/SIGNIFICANCE: Because of the geographical divide of the mycobacterial populations in West Africa, individual research findings from one country cannot be generalized across the whole region. The unequal geographical family distribution should be considered in placement and design of future clinical trials in West Africa. en_US
dc.language English en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis complex en_US
dc.subject Mycobacterium africanum en_US
dc.subject Molecular epidemiology en_US
dc.subject Population structure en_US
dc.subject Phylogeography en_US
dc.subject Genotyping en_US
dc.subject Spoligotyping en_US
dc.subject Geographical variation en_US
dc.subject Africa, West en_US
dc.title A Mycobacterial perspective on tuberculosis in West Africa: significant geographical variation of M. africanum and other M. tuberculosis complex lineages en_US
dc.type Article-E en_US
dc.citation.issue 3 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.volume 10 en_US
dc.citation.pages e0004408 en_US
dc.citation.jabbreviation PLoS Negl Trop Dis en_US

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