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Prospective evaluation and comparison of the direct agglutination test and an rK39-antigen-based dipstick test for the diagnosis of suspected kala-azar in Nepal

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dc.contributor.author Chappuis, F. en_US
dc.contributor.author Rijal, S. en_US
dc.contributor.author Singh, R. en_US
dc.contributor.author Acharya, P. en_US
dc.contributor.author Karki, B. M. S. en_US
dc.contributor.author Das, M. L. en_US
dc.contributor.author Bovier, P. A. en_US
dc.contributor.author Desjeux, P. en_US
dc.contributor.author Le Ray, D. en_US
dc.contributor.author Koirala, S. en_US
dc.contributor.author Loutan, L. en_US
dc.date.accessioned 2007-12-06T14:33:15Z
dc.date.available 2007-12-06T14:33:15Z
dc.date.issued 2003 en_US
dc.identifier.issn 1360-2276 en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-3156.2003.01026.x
dc.identifier.other ITG-P9A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTB en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/89
dc.description The definitive version is available at www3.interscience.wiley.com
dc.description.abstract The diagnosis of visceral leishmaniasis (kala-azar) remains difficult in rural endemic areas and practical and reliable tests are badly needed. Two serological tests, the Direct Agglutination Test (DAT) and an rK39-antigen-based dipstick test, were compared to parasitological diagnosis in a group of 184 patients presenting at a tertiary care centre in south-eastern Nepal with a history of fever ≥14 days and splenomegaly; 139 patients had a parasitologically proven kala-azar and 45 patients had a negative parasitological work-up. The rK39 dipstick showed a sensitivity of 97% and a specificity of 71%. The DAT was up to 99% sensitive with a low cut-off titre (1:400) but its specificity did not exceed 82% even with a high cut-off titre (1:51 200). Both tests could be used for screening suspect patients in endemic areas. However, their use as confirmatory tests should be restricted to situations where the proportion of kala-azar among clinical suspect patients is high. The rK39 dipstick is cheaper and easier to use than the DAT and could be used widely provided that both its performance and production remain stable. en_US
dc.language English en_US
dc.publisher Blackwell Publishing
dc.subject Protozoal diseases en_US
dc.subject Leishmaniasis en_US
dc.subject Visceral en_US
dc.subject Kala azar en_US
dc.subject Laboratory diagnosis en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Agglutination tests en_US
dc.subject Dipstick en_US
dc.subject k39 antigen en_US
dc.subject Tests en_US
dc.subject Evaluation en_US
dc.subject Comparative study en_US
dc.subject Nepal en_US
dc.subject Asia, South en_US
dc.title Prospective evaluation and comparison of the direct agglutination test and an rK39-antigen-based dipstick test for the diagnosis of suspected kala-azar in Nepal en_US
dc.type Article en_US
dc.citation.issue 3
dc.citation.jtitle Tropical Medicine and International Health en_US
dc.citation.volume 8 en_US
dc.citation.pages 277-285 en_US
dc.publisher.place Oxford
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/12631320
dc.citation.jabbreviation Trop Med Int Health en_US


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