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Validation of primary CD4 gating as an affordable strategy for absolute CD4 counting in Cambodia

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Show simple item record Lynen, L. en_US Teav, S. en_US Vereecken, C. en_US De Munter, P. en_US An, S. en_US Jacques, G. en_US Kestens, L. en_US 2007-12-06T14:38:51Z 2007-12-06T14:38:51Z 2006 en_US
dc.identifier.issn 1525-4135 en_US
dc.identifier.other ITG-C1A en_US
dc.identifier.other ITG-M3B en_US
dc.identifier.other ITG-C4B en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other CLINIC en_US
dc.identifier.other U-HIVCLI en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-IMMUN en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.description Not the final published version en_US
dc.description.abstract OBJECTIVE: To validate primary CD4 gating in lysed whole blood for absolute CD4 counts in fresh and aged blood using an affordable compact volumetric commercial flow cytometer. DESIGN: Comparison of CD4 counts between the FACSCount and the 2-parameter CyFlow SL Green. METHODS: One hundred twenty fresh blood samples from patients likely to be infected with HIV were simultaneously run on a FACSCount at the Pasteur Institute of Cambodia and on a CyFlow SL Green at the Sihanouk Hospital Center of Hope (SHCH), Phnom Penh, Cambodia. Intra- and interrun precision was assessed using 2 blood samples. Stability of CD4 counting in blood stored up to 96 hours at room temperature was assessed using 27 blood samples. RESULTS: CD4 counts on the CyFlow SL Green and on the FACSCount correlated well apart from a relative bias (R = 0.993, bias of -9.5%, 95% confidence interval [CI]: -11.8% to -7.1%, limits of agreement: -32.5% to 13.6%). Intra- and interrun variability ranged from 3% to 5% and from 5% to 6%, respectively. CD4 counts on aged blood using the CyFlow SL Green showed an interassay variability of <10%. CONCLUSIONS: Primary CD4 gating in lysed whole blood using the CyFlow SL Green is an affordable and precise method for CD4 counting. Because the fluorescence (FL) and light scatter signals have to be analyzed manually, however, intensive training of the technician and/or operator is imperative. en_US
dc.language English en_US
dc.publisher Lippincott, Williams & Wilkins en_US
dc.subject Viral diseases en_US
dc.subject HIV en_US
dc.subject AIDS en_US
dc.subject Monitoring en_US
dc.subject CD4 lymphocyte count en_US
dc.subject Flow cytometry en_US
dc.subject Affordability en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Developing countries en_US
dc.title Validation of primary CD4 gating as an affordable strategy for absolute CD4 counting in Cambodia en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Journal of Acquired Immune Deficiency Syndromes en_US
dc.citation.volume 43 en_US
dc.citation.pages 179-185 en_US Philadelphia en_US
dc.citation.jabbreviation J Acquir Immun Defic Syndr en_US

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