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A comparative test of ixodid tick identification by a network of European researchers

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Show simple item record Estrada-Pena, A. en_US D'Amico, G. en_US Palomar, A. M. en_US Dupraz, M. en_US Fonville, M. en_US Heylen, D. en_US Habela, M. A. en_US Hornok, S. en_US Lempereur, L. en_US Madder, M. en_US Nuncio, M. S. en_US Otranto, D. en_US Pfaffle, M. en_US Plantard, O. en_US Santos-Silva, M. M. en_US Sprong, H. en_US Vatansever, Z. en_US Vial, L. en_US Mihalca, A. D. en_US 2017-12-18T12:55:40Z 2017-12-18T12:55:40Z 2017 en_US
dc.identifier.issn 1877-959X en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 34 en_US
dc.identifier.other 34 en_US
dc.identifier.other ITG-B10X; DBM; UVENTOM: JIF; DOI; PDF; Abstract; DSPACE64 en_US
dc.description.abstract This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, Hyalomma lusitanicum, Hy. marginatum, Ixodes ricinus, I. hexagonus, Rhipicephalus annulatus, R. bursa, R. rossicus, and/or R. sanguineus s.l. Ticks were morphologically identified by three of the co-authors and the identification confirmed by a fourth co-author who used molecular methods based on several genes. Then ticks were randomly selected and blindly distributed among participants, together with a questionnaire. Only specimens collected while questing and, if possible, in the same survey, were circulated. Because of the random nature of the test, a participant could receive several specimens of the same species. Species in the different genera had variable misidentification rates (MR) of 7% (Dermacentor), 14% (Ixodes), 19% (Haemaphysalis), 36% (Hyalomma), and 54% (Rhipicephalus). Within genera, the MR was also variable ranging from 5.4% for I. ricinus or 7.4% for D. marginatus or D. reticulatus to 100% for R. rossicus. The test provided a total misidentification rate of 29.6% of the species of ticks. There are no significant differences in MR according to the sex of the tick. Participants were requested to perform a second round of identifications on the same set of ticks, using only purposely prepared keys (without illustrations), circulated to the enrolled participants, including 2 species of the genus Dermacentor, 8 of Haemaphysalis, 10 of Hyalomma, 23 of Ixodes, and 6 of Rhipicephalus. The average MR in the second round was 28%: 0% (Dermacentor), 33% (Haemaphysalis), 30% (Hyalomma) 18% (Ixodes), and 50% (Rhipicephalus). Species which are not reported in the countries of a participating laboratory had always highest MR, i.e. purely Mediterranean species had highest MR by laboratories in Central and Northern Europe. Participants expressed their concerns about a correct identification for almost 50% of the ticks of the genera Hyalomma and Rhipicephalus. The results revealed less than total confidence in identifying the most prominent species of ticks in the Western Palearctic, and underpin the need for reference libraries for specialists involved in this task. Results also showed that a combination of certain genes may adequately identify the target species of ticks. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Ticks en_US
dc.subject Entomology en_US
dc.subject Identification en_US
dc.subject Ixodes en_US
dc.subject Rhipicephalus en_US
dc.subject Hyalomma en_US
dc.subject Europe-West en_US
dc.subject Africa-North en_US
dc.title A comparative test of ixodid tick identification by a network of European researchers en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Ticks and Tick-Borne Diseases en_US
dc.citation.volume 8 en_US
dc.citation.pages 540-546 en_US
dc.citation.abbreviation Ticks Tick Borne Dis en_US

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