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High prevalence of bovine cysticercosis found during evaluation of different post-mortem detection techniques in Belgian slaughterhouses

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dc.contributor.author Jansen, F. en_US
dc.contributor.author Dorny, P. en_US
dc.contributor.author Berkvens, D. en_US
dc.contributor.author Van Hul, A. en_US
dc.contributor.author Van den Broeck, N. en_US
dc.contributor.author Makay, C. en_US
dc.contributor.author Praet, N. en_US
dc.contributor.author Eichenberger, R. M. en_US
dc.contributor.author Deplazes, P. en_US
dc.contributor.author Gabriël, S. en_US
dc.date.accessioned 2017-12-18T12:55:43Z
dc.date.available 2017-12-18T12:55:43Z
dc.date.issued 2017 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.vetpar.2017.07.009 en_US
dc.identifier.other 6 pp. en_US
dc.identifier.other ITG-B1A; ITG-B2A; ITG-B3A; ITG-B4A; ITG-B5A; ITG-B6B; ITG-B7X; ITG-BLA; DBM; U-VHELM; U-VEPID; JIF; DOI; CPDF; Abstract; DSPACE64 en_US
dc.identifier.uri http://hdl.handle.net/10390/9543
dc.description.abstract Bovine cysticercosis (BCC), caused by the helminth Taenia saginata, is currently diagnosed solely by official meat inspection (MI) based on macroscopic detection of viable cysticerci or typical lesions of degenerated larvae. MI has a known low sensitivity (<16%), leading to a large proportion of infected cattle carcasses entering the human food chain and posing a risk to public health. Prevalence in Belgium based on MI results is estimated at around 0.22%. Due to the low sensitivity of MI, alternative techniques to detect BCC should be considered. This study evaluates MI, MI with additional incisions in the heart, specific antibody detection against excretory/secretory (E/S) in the Ab-ELISA and circulating antigens in the B158/B60 Ag-ELISA on 715 (101 MI-positive and 614 MI-negative) samples collected from carcasses at slaughterhouses in Belgium. Full dissection of the predilection sites was considered the reference test. During the study, mostly carcasses with (very) light infections were detected containing predominantly degenerated or calcified cysticerci and only few viable cysticerci. Dissection of the predilection sites detected 144 (23%) additional infections in the 614 MI-negative carcasses. When sequentially performing first the dissection of the predilection sites, followed by the Ag-ELISA and the Ab-ELISA, an additional 36% of MI-negative carcasses were found positive for BCC, resulting in a prevalence very much higher than the above mentioned 0.22%. The B158/B60 Ag-ELISA showed a sensitivity of 40% for the detection of carcasses containing viable cysticerci and a specificity of 100%, and detected 70 positive carcasses of which only 14 had been identified as positive during MI. If Ag-ELISA were implemented as a detection technique for BCC in the slaughterhouses, many infected carcasses would still not be detected due to the sensitivity of 40%. But as sensitivity increases with increasing number of cysticerci in the carcass, the infected carcasses passing inspection will be the ones containing only a few viable cysticerci and thus posing a smaller food safety problem. Ag-ELISA is preferred over the ES Ab-ELISA in this study, which had a sensitivity of 13.3% and a specificity of 91.7% in a population with overall low infection burdens. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/28917298 en_US
dc.subject Cysticercosis en_US
dc.subject Helminthic diseases en_US
dc.subject Animal diseases en_US
dc.subject Cattle en_US
dc.subject Belgium en_US
dc.subject Europe-West en_US
dc.title High prevalence of bovine cysticercosis found during evaluation of different post-mortem detection techniques in Belgian slaughterhouses en_US
dc.type Article en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 244 en_US
dc.citation.pages 1-6 en_US
dc.citation.abbreviation Vet Parasitol en_US


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