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Evaluation of an automated quantitative latex immunoturbidimetric non-treponemal assay for diagnosis and follow-up of syphilis: a prospective cohort study

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dc.contributor.author Osbak, K. en_US
dc.contributor.author Abdellati, S. en_US
dc.contributor.author Tsoumanis, A. en_US
dc.contributor.author Van Esbroeck, M. en_US
dc.contributor.author Kestens, L. en_US
dc.contributor.author Crucitti, T. en_US
dc.contributor.author Kenyon, C. en_US
dc.date.accessioned 2017-12-18T12:55:48Z
dc.date.available 2017-12-18T12:55:48Z
dc.date.issued 2017 en_US
dc.identifier.issn 0022-2615 en_US
dc.identifier.doi http://dx.doi.org/10.1099/jmm.0.000559 en_US
dc.identifier.other http://lib.itg.be/pdf/itg/2017/2017jmmi1130.pdf en_US
dc.identifier.other ITG-C1A; ITG-C2B; ITG-C3B; ITG-C4A; ITG-B5A; ITG-C6A; ITG-CLA; MULTI; DCS; DBM; U-xxx; U-IMMUN; JIF; DOI; PDF; Abstract; DSPACE64 en_US
dc.identifier.uri http://hdl.handle.net/10390/9607
dc.description.abstract PURPOSE: We evaluated the Sekure rapid plasma reagin (RPR-S) (Sekisui Diagnostics) automated quantitative latex immunoturbidimetric assay performed on the SK500 clinical chemistry system for clinical appropriateness. METHODOLOGY: Syphilis-infected individuals and controls were recruited into a prospective cohort study conducted at a sexually transmitted infection clinic in Antwerp, Belgium. Sera collected at diagnosis (baseline) and at 3, 6, 9 and 12 months post-treatment were tested with RPR-S and Macro-Vue RPR card (RPR-C) (Becton Dickinson) assays; RPR-C was considered the reference test. IgG/IgM enzyme immunoassay and Treponema pallidum polA serum PCR results were consulted by discordancy at baseline. Categorical analyses were performed and correlations were assessed with (non)-linear regression. Post-treatment longitudinal serological evolution was evaluated. RESULTS: A total of 463 samples from 120 new syphilis cases from a variety of clinical stages and 30 syphilis-negative controls were tested. Initially, there was a weak correlation between quantitative RPR-C/S (r=0.15). In 70 samples there was a strong suspicion of hook effect. Of these, 57/70 sera were retested with an extra dilution step, resulting in an average 12-fold increase in quantitative RPR-S results. After the extra dilution, the overall qualitative RPR-C/S agreement was 78.89 %, (kappa-coefficient: 0.484). Of the 92 discordant samples, 9 were from the baseline visit (RPR-C titre: 1-8), which could have led to possible missed diagnoses using the RPR-S. CONCLUSIONS: The sensitivity and accuracy of the RPR-S test requires improvement before it can be used to diagnose syphilis and evaluate treatment efficacy in clinical practice. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/28792374 en_US
dc.subject Syphilis en_US
dc.subject Bacterial diseases en_US
dc.subject Sexually transmitted diseases en_US
dc.subject STD en_US
dc.subject Diagnostic tests en_US
dc.subject Belgium en_US
dc.subject Europe-West en_US
dc.title Evaluation of an automated quantitative latex immunoturbidimetric non-treponemal assay for diagnosis and follow-up of syphilis: a prospective cohort study en_US
dc.type Article-P en_US
dc.citation.jtitle Journal of Medical Microbiology en_US
dc.citation.volume [Epub ahead of print] en_US
dc.citation.abbreviation J Med Microbiol en_US


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