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Whole-genome sequencing illuminates the evolution and spread of multidrug-resistant tuberculosis in Southwest Nigeria

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Show simple item record Senghore, M. en_US Otu, J. en_US Witney, A. en_US Gehre, F. en_US Doughty, E. L. en_US Kay, G. L. en_US Butcher, P. en_US Salako, K. en_US Kehinde, A. en_US Onyejepu, N. en_US Idigbe, E. en_US Corrah, T. en_US De Jong, B. en_US Pallen, M. J. en_US Antonio, M. en_US 2017-12-18T12:55:50Z 2017-12-18T12:55:50Z 2017 en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 12 pp. en_US
dc.identifier.other ITG-B4A; ITG-B13A; DBM; U-MYCOB; JIF; OAJ; DOI; PDF; PMC; Abstract; DSPACE64 en_US
dc.description.abstract Nigeria has an emerging problem with multidrug-resistant tuberculosis (MDR-TB). Whole-genome sequencing was used to understand the epidemiology of tuberculosis and genetics of multi-drug resistance among patients from two tertiary referral centers in Southwest Nigeria. In line with previous molecular epidemiology studies, most isolates of Mycobacterium tuberculosis from this dataset belonged to the Cameroon clade within the Euro-American lineage. Phylogenetic analysis showed this clade was undergoing clonal expansion in this region, and suggests that it was involved in community transmission of sensitive and multidrug-resistant tuberculosis. Five patients enrolled for retreatment were infected with pre-extensively drug resistant (pre-XDR) due to fluoroquinolone resistance in isolates from the Cameroon clade. In all five cases resistance was conferred through a mutation in the gyrA gene. In some patients, genomic changes occurred in bacterial isolates during the course of treatment that potentially led to decreased drug susceptibility. We conclude that inter-patient transmission of resistant isolates, principally from the Cameroon clade, contributes to the spread of MDR-TB in this setting, underscoring the urgent need to curb the spread of multi-drug resistance in this region. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Tuberculosis-multidrug-resistant en_US
dc.subject Bacterial diseases en_US
dc.subject Epidemiology en_US
dc.subject Nigeria en_US
dc.subject Africa-West en_US
dc.title Whole-genome sequencing illuminates the evolution and spread of multidrug-resistant tuberculosis in Southwest Nigeria en_US
dc.type Article-E en_US
dc.citation.issue 9 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.volume 12 en_US
dc.citation.pages e0184510 en_US
dc.citation.abbreviation PLoS ONE en_US

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