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Comparison of leishmania typing results obtained from 16 European clinical laboratories in 2014

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dc.contributor.author Van der Auwera, G. en_US
dc.contributor.author Bart, A. en_US
dc.contributor.author Chicharro, C. en_US
dc.contributor.author Cortes, S. en_US
dc.contributor.author Davidsson, L. en_US
dc.contributor.author Di Muccio, T. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.contributor.author Felger, I. en_US
dc.contributor.author Paglia, M. G. en_US
dc.contributor.author Grimm, F. en_US
dc.contributor.author Harms, G. en_US
dc.contributor.author Jaffe, C. L. en_US
dc.contributor.author Manser, M. en_US
dc.contributor.author Ravel, C. en_US
dc.contributor.author Robert-Gangneux, F. en_US
dc.contributor.author Roelfsema, J. en_US
dc.contributor.author Toz, S. en_US
dc.contributor.author Verweij, J. J. en_US
dc.contributor.author Chiodini, P. L. en_US
dc.date.accessioned 2017-12-18T12:56:09Z
dc.date.available 2017-12-18T12:56:09Z
dc.date.issued 2016 en_US
dc.identifier.issn 1025-496X en_US
dc.identifier.doi http://dx.doi.org/10.2807/1560-7917.ES.2016.21.49.30418 en_US
dc.identifier.other http://lib.itg.be/pdf/itg/2016/2016esur30418.pdf en_US
dc.identifier.other 33 en_US
dc.identifier.other ITG-B1A; ITG-B7A; DBM; U-MOLPAR; JIF; DOI; PDF; PMC; Abstract; DSPACE64 en_US
dc.identifier.uri http://hdl.handle.net/10390/9914
dc.description.abstract Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/27983510 en_US
dc.subject Leishmania en_US
dc.subject Protozoology en_US
dc.subject Typing en_US
dc.subject Laboratory medicine en_US
dc.subject Europe en_US
dc.title Comparison of leishmania typing results obtained from 16 European clinical laboratories in 2014 en_US
dc.type Article en_US
dc.citation.issue 49 en_US
dc.citation.jtitle Eurosurveillance en_US
dc.citation.volume 21 en_US
dc.citation.pages 1 en_US
dc.citation.abbreviation Euro Surveill en_US


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