Institute of Tropical Medicine Antwerp
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Comparison of leishmania typing results obtained from 16 European clinical laboratories in 2014

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Show simple item record Van der Auwera, G. en_US Bart, A. en_US Chicharro, C. en_US Cortes, S. en_US Davidsson, L. en_US Di Muccio, T. en_US Dujardin, J. C. en_US Felger, I. en_US Paglia, M. G. en_US Grimm, F. en_US Harms, G. en_US Jaffe, C. L. en_US Manser, M. en_US Ravel, C. en_US Robert-Gangneux, F. en_US Roelfsema, J. en_US Toz, S. en_US Verweij, J. J. en_US Chiodini, P. L. en_US 2017-12-18T12:56:09Z 2017-12-18T12:56:09Z 2016 en_US
dc.identifier.issn 1025-496X en_US
dc.identifier.doi en_US
dc.identifier.other en_US
dc.identifier.other 33 en_US
dc.identifier.other ITG-B1A; ITG-B7A; DBM; U-MOLPAR; JIF; DOI; PDF; PMC; Abstract; DSPACE64 en_US
dc.description.abstract Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally. en_US
dc.language English en_US
dc.relation.uri en_US
dc.subject Leishmania en_US
dc.subject Protozoology en_US
dc.subject Typing en_US
dc.subject Laboratory medicine en_US
dc.subject Europe en_US
dc.title Comparison of leishmania typing results obtained from 16 European clinical laboratories in 2014 en_US
dc.type Article en_US
dc.citation.issue 49 en_US
dc.citation.jtitle Eurosurveillance en_US
dc.citation.volume 21 en_US
dc.citation.pages 1 en_US
dc.citation.abbreviation Euro Surveill en_US

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