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Hepatitis B virus genotypes A and D in Uganda

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dc.contributor.author Zirabamuzale, J. T. en_US
dc.contributor.author Opio, C. K. en_US
dc.contributor.author Bwanga, F. en_US
dc.contributor.author Seremba, E. en_US
dc.contributor.author Apica, B. S. en_US
dc.contributor.author Colebunders, R. en_US
dc.contributor.author Ocama, P. en_US
dc.date.accessioned 2017-12-18T12:56:11Z
dc.date.available 2017-12-18T12:56:11Z
dc.date.issued 2016 en_US
dc.identifier.issn 2055-6640 en_US
dc.identifier.other http://lib.itg.be/pdf/itg/2016/2016jver0019.pdf en_US
dc.identifier.other 17 en_US
dc.identifier.other ITG-C6A; DPH; U-HIVCLI; PDF; PMC; Abstract; DSPACE64 en_US
dc.identifier.uri http://hdl.handle.net/10390/9940
dc.description.abstract BACKGROUND: The prevalence of hepatitis B virus (HBV) infection in Uganda is 10%. Hepatitis B virus genotypes impact on treatment response, rate of spontaneous recovery and progression of chronic HBV infection and hepatocellular carcinoma. There is little information on the HBV genotypic distribution in Uganda. OBJECTIVES: To determine HBV genotypes in Uganda. METHODS: The MBN clinical laboratory performs HBV viral load and genotype testing in Uganda. It receives hepatitis B surface antigen (HBsAg)-positive samples from all over the country for additional HBV testing. Samples are stored for 6 months before being discarded. Our study used delinked stored samples. PCR-positive samples had DNA extracted and used as template for HBV genome amplification by nested PCR. Reverse hybridisation was performed and genotypes were determined by the line probe assay method (INNO-LiPA). RESULTS: One hundred stored HBsAg-positive plasma samples with detectable viral loads were analysed. Of these, 93 samples showed PCR amplification products and gave genotype-specific probe lines on the INNO-LiPA assay. Of the patients, where gender was recorded, 60.9% were female, and the overall median age (IQR) was 25 (2-60) years. There was a predominance of HBV genotype D (47 patients; 50.5%), followed by genotype A, (16 patients; 17.2%). One patient (1.1%) had genotype E. In 28% of the samples mixed infections were detected with genotypes A/E (9.7%) and A/D (6.5%) being most common. Genotypes B, C, E and H only occurred as part of mixed infections. CONCLUSION: Hepatitis B genotypes D and A were predominant in our study population. en_US
dc.language English en_US
dc.publisher Journal of Virus Eradication en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/27482430 en_US
dc.subject Hepatitis B en_US
dc.subject Viral diseases en_US
dc.subject Virology en_US
dc.subject Genotypes en_US
dc.subject Uganda en_US
dc.subject Africa-East en_US
dc.title Hepatitis B virus genotypes A and D in Uganda en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.volume 2 en_US
dc.citation.pages 19 en_US
dc.citation.abbreviation J Virus Erad en_US


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