Institute of Tropical Medicine Antwerp
Foundation of Public Utility

Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Guha, R. en_US
dc.contributor.author Das, S. en_US
dc.contributor.author Ghosh, J. en_US
dc.contributor.author Sundar, S. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.contributor.author Roy, S. en_US
dc.date.accessioned 2017-12-18T12:56:11Z
dc.date.available 2017-12-18T12:56:11Z
dc.date.issued 2014 en_US
dc.identifier.issn 1935-2727 en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0002995 en_US
dc.identifier.other http://lib.itg.be/pdf/itg/2014/2014pntde2995.pdf en_US
dc.identifier.other 11 pp.; Address incorrect: UA en_US
dc.identifier.other 62 en_US
dc.identifier.other ITG-N5A; DBM; U-MOLPAR; JIF; DOI; PDF; Abstract; OAJ; E-only; DSPACE64 en_US
dc.identifier.uri http://hdl.handle.net/10390/9948
dc.description.abstract In India the sand fly, Phlebotomus argentipes, transmitted parasitic disease termed kala-azar is caused by Leishmania donovani (LD) in humans. These immune-evading parasites have increasingly developed resistance to the drug sodium antimony gluconate in endemic regions. Lack of early diagnosis methods for the disease limits the information available regarding the early interactions of this parasite with either human tissues or cell lineages. We reasoned that peripheral blood mononuclear cells (PBMCs) from healthy human beings could help compare some of their immune signatures once they were exposed for up to 8 days, to either pentavalent antimony sensitive (SbS-LD) or resistant (SbR-LD) Leishmania donovani isolates. At day 2, PBMC cultures exposed to SbS-LD and SbR-LD stationary phase promastigotes had four and seven fold higher frequency of IL-10 secreting monocyte-macrophage respectively, compared to cultures unexposed to parasites. Contrasting with the CD4+CD25-C. en_US
dc.language English en_US
dc.relation.uri http://www.ncbi.nlm.nih.gov/pubmed/25032977 en_US
dc.subject Protozoal diseases en_US
dc.subject Kala azar en_US
dc.subject Visceral en_US
dc.subject Leishmaniasis en_US
dc.subject Leishmania donovani en_US
dc.subject Vectors en_US
dc.subject Sandflies en_US
dc.subject Phlebotomus argentipes en_US
dc.subject Drug resistance en_US
dc.subject Immune response en_US
dc.subject T cells en_US
dc.subject Regulation en_US
dc.subject Interactions en_US
dc.subject Frequency en_US
dc.subject Peripheral blood mononuclear cell (PBMC) en_US
dc.subject IL-10 en_US
dc.subject TGF-beta en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject India en_US
dc.subject Asia-South en_US
dc.title Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response en_US
dc.type Article-E en_US
dc.citation.issue 7 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.volume 8 en_US
dc.citation.pages e2995 en_US
dc.citation.abbreviation PLoS Negl Trop Dis en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record