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The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum

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dc.contributor.author Claes, F. en_US
dc.contributor.author Verloo, D. en_US
dc.contributor.author De Waal, D. T. en_US
dc.contributor.author Majiwa, P. A. O. en_US
dc.contributor.author Baltz, T. en_US
dc.contributor.author Goddeeris, B. M. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:33:17Z
dc.date.available 2007-12-06T14:33:17Z
dc.date.issued 2003 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi http://dx.doi.org/10.1016/S0304-4017(02)00359-X
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P2A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/99
dc.description.abstract In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T. evansi and T. equiperdum, respectively, was analyzed. Ten strains of T. evansi and 11 strains of T. equiperdum originating from Asia, Europe, Africa and Latin America were tested. Rabbit pre-infection sera and sera of days 7, 14, 25, 35 post-infection (p.i.) were analyzed for the presence of antibodies reactive with RoTat 1.2 in immune trypanolysis, ELISA/T. evansi and CATT/T. evansi. Within the duration of the infection (maximum 35 days), all T. evansi as well as 9 out of 11 T. equiperdum infected rabbits became positive in all these tests. The rabbits infected with T. equiperdum OVI (South Africa) and BoTat 1.1 (Morocco) remained negative in the immune trypanolysis test although the latter rabbit became positive in the CATT/T. evansi and ELISA/T. evansi. On the contrary, both rabbits were positive in immune trypanolysis when tested against their respective infecting population. From these data, we conclude that most T. equiperdum strains express isoVATs of RoTat 1.2. This explains, in part, why antibody tests based on T. evansi RoTat 1.2 cannot reliably distinguish between infections caused by T. evansi and those caused by T. equiperdum unless it can be proven that most described T. equiperdum are actually misclassified T. evansi. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoology en_US
dc.subject Dourine en_US
dc.subject Surra en_US
dc.subject Trypanosoma equiperdum en_US
dc.subject Trypanosoma evansi en_US
dc.subject Strains en_US
dc.subject Characterization en_US
dc.subject Antibody detection en_US
dc.subject Variant surface glycoproteins en_US
dc.subject RoTat 1.2 en_US
dc.subject Specificity en_US
dc.title The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum en_US
dc.type Article en_US
dc.citation.issue Sensitivity en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 116 en_US
dc.citation.pages 209-216 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/14559163
dc.identifier.url http://www.elsevier.com/locate/vetpar
dc.citation.jabbreviation Vet Parasitol en_US


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